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Updated: May 23, 2025

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
Curcumin-loaded platelets with hydroxypropyl chitin hydrogel composite system through photo-activation for effective
Xiang-Yi Zhao1, Jing-Yu Lu2, Liang Zheng3
1Shiyan Key Laboratory of Natural Medicine Nanoformulation Research, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Renmin road No. 30, Shiyan, Hubei 442000, China.
Abstract:
Platelet-mediated wound healing represents a complex process for tissue repair and regeneration. This study investigates a novel therapeutic approach that combines curcumin-loaded platelets (PLT/CUR) with hydroxypropyl chitin hydrogel (PLT/CUR/HPCH) to enhance wound healing through photo-activation. PLT/CUR was characterized by flow cytometry, laser scanning confocal microscopy, and transmission electron microscopy. The efficacy of PLT/CUR/HPCH was evaluated using two hemorrhage models in New Zealand white rabbits and acute trauma rats. The results demonstrated successful CUR loading into PLT without compromising their viability. Laser irradiation facilitated PLT aggregation and expression of CD62p/TXA2R through COX-2/reactive oxygen species bursts. The activated PLT further encouraged the proliferation and migration of endothelium with faster in vitro angiogenesis. PLT/CUR/HPCH significantly reduced bleeding time and blood loss in rabbit ear marginal vein and liver hemorrhage models, while exhibiting antimicrobial activity against Escherichia coli and Staphylococcus aureus. Furthermore, the composite hydrogel demonstrated robust healing efficacy in the rat acute wound model. Notably, the photo-activated PLT/CUR/HPCH group exhibited a wound closure rate of approximately 53 % on the third day, while the control group had <8 %. Therefore, this work establishes a theoretical framework for targeted PLT activation in wound repair and introduces novel applications for PLT-based biopharmaceuticals.
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