Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.7K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.7K
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

3.4K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.4K
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

3.7K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.7K
Post-translational Translocation of Proteins to the RER01:27

Post-translational Translocation of Proteins to the RER

5.5K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
5.5K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

4.3K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.3K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.3K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

I-tRF-Asp Promotes Vascular Remodeling in Hypoxic Pulmonary Hypertension Via hnRNPU Phase Separation, Which Affects TCF7L2 Alternative Splicing.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

tsRNA-3040b accumulates R-loop to regulate Trim35 transcription, which leads to disordered glycolysis and promotes PAECs proliferation.

Cellular & molecular biology letters·2025
Same author

LINC00599 Promotes Pulmonary Hypertension via Liquid-Liquid Phase Separation With G3BP1 and MYH9.

Hypertension (Dallas, Tex. : 1979)·2025
Same author

Circ-myh8/KAT7 Affects PANoptosis in Pulmonary Arterial Smooth Muscle Cells: Involvement of Super-Enhancers in FOSL2 Expression.

Journal of the American Heart Association·2025
Same author

Super-Enhancer-Driven HCG20 Promotes Pulmonary Hypertension Through U2AF2 Splicing.

Circulation research·2025
Same author

Mitochondrial Genome-Encoded lncND5 Regulates Mitophagy in Hypoxic Pulmonary Artery Smooth Muscle Cell.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same journal

Brain Iron in Nucleus Accumbens and Cognitive Function in Preeclampsia.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Hypertension Treatment Intentions: A Health Belief Model Analysis.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Changes in Retinal Microvasculature During Healthy Pregnancy Measured by AO.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Longitudinal Maternal IgG and IgA Glycosylation Profiles in Pregnancy Reveal Early Immune Alterations in Placenta-Related Complications: The Rotterdam Periconception Cohort.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Blood Pressure Variability and Outcomes Across Antihypertensive Regimens.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Rural Health and Health Disparities in Hypertension Management: A Scientific Statement From the American Heart Association.

Hypertension (Dallas, Tex. : 1979)·2026
See all related articles

Related Experiment Video

Updated: May 20, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

808

ca-circSCN8A Promotes HPASMCs Ferroptosis via LLPS Initiated R-Loop.

Mengnan Li1,2,3, Yingying Hao1,3, Xinyue Song1,3

  • 1College of Pharmacy (M.L., Y.H., X.S., H.L., H.S., L.Z., H.Y., C.M., X. Zhao, D.Z.), Harbin Medical University, PR China.

Hypertension (Dallas, Tex. : 1979)
|May 19, 2025
PubMed
Summary
This summary is machine-generated.

Chromatin-associated RNA circSCN8A promotes ferroptosis in pulmonary hypertension by forming R-loops with the SLC7A11 promoter, offering a potential therapeutic target.

Keywords:
R-loop structuresRNA-binding protein FUSferroptosishypertension, pulmonaryphase separation

More Related Videos

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
07:14

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Published on: February 25, 2022

5.9K
Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts
09:21

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

Published on: February 23, 2024

800

Related Experiment Videos

Last Updated: May 20, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

808
Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
07:14

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Published on: February 25, 2022

5.9K
Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts
09:21

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

Published on: February 23, 2024

800

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Ferroptosis is implicated in pulmonary hypertension (PH).
  • Chromatin-associated RNAs (ca-RNAs) regulate ferroptosis, but mechanisms are unclear.
  • This study investigates ca-circSCN8A's role in PH-related ferroptosis.

Purpose of the Study:

  • To identify and characterize the function of ca-circSCN8A in hypoxia-induced ferroptosis in pulmonary arterial smooth muscle cells.
  • To elucidate the molecular mechanism by which ca-circSCN8A regulates ferroptosis in PH.
  • To explore ca-circSCN8A as a potential therapeutic target for PH.

Main Methods:

  • Bioinformatics, Sanger sequencing, and RNase R digestion identified ca-circSCN8A upregulation.
  • Functional gain and loss assays assessed ca-circSCN8A's role in ferroptosis in vitro and in vivo.
  • RNA immunoprecipitation, pull-down assays, and various molecular assays explored the interaction with FUS and the mechanism of action.

Main Results:

  • ca-circSCN8A was upregulated in PH and promoted hypoxia-induced ferroptosis.
  • ca-circSCN8A recruited EP300 to lactylate FUS, forming a complex via liquid-liquid phase separation.
  • This complex stabilized an R-loop with the SLC7A11 promoter, inhibiting transcription and disrupting redox homeostasis.

Conclusions:

  • ca-circSCN8A drives ferroptosis in PH by forming R-loops with nonhost gene promoters via liquid-liquid phase separation.
  • This study reveals a novel mechanism of circRNA-mediated gene regulation.
  • ca-circSCN8A is a key regulator and potential therapeutic target for ferroptosis in hypoxic PH.