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Identification of an Anoikis-associated LncRNA Signature to Predict the Clinical Prognosis and Immune Function of Patients with Endometrial Cancer

  • 0Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
International Journal of Medical Sciences +

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May 19, 2025

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May 19, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study identifies seven anoikis-associated long non-coding RNAs (lncRNAs) as key prognostic markers for endometrial cancer. These markers can predict patient outcomes and guide immunotherapy strategies, offering new therapeutic targets for this disease.

Area Of Science

  • Oncology
  • Molecular Biology
  • Genomics

Background

  • Endometrial cancer is a heterogeneous malignancy with high mortality and poor prognosis in advanced stages.
  • Anoikis, a form of programmed cell death, plays a critical role in cancer development and metastasis.
  • The prognostic and therapeutic implications of anoikis-associated long non-coding RNAs (lncRNAs) in endometrial cancer are not well understood.

Purpose Of The Study

  • To develop a novel prognostic signature for endometrial cancer based on anoikis-related lncRNAs.
  • To investigate the association of these lncRNAs with tumor progression, immune function, and drug sensitivity.
  • To validate the function of a specific lncRNA, CFAP58-DT, in endometrial cancer development.

Main Methods

  • Utilized The Cancer Genome Atlas (TCGA) database for transcriptome sequencing and clinical data.
  • Employed multivariate regression and least absolute shrinkage and selection operator (LASSO) regression to construct a prognostic signature.
  • Validated the signature using receiver operating characteristic (ROC) curve and Kaplan-Meier analyses; performed gene set enrichment analysis (GSEA) and in vitro/in vivo experiments.

Main Results

  • Identified seven anoikis-associated lncRNAs (CFAP58-DT, AC004585.1, AC103563.9, AL121895.2, AC004596.1, AC010761.4, AC087564.1) with significant prognostic value.
  • The developed signature demonstrated excellent predictive accuracy (AUC = 0.757) and revealed potential roles in cellular processes and immune evasion.
  • Silencing CFAP58-DT inhibited proliferation, promoted apoptosis, and reduced tumor malignancy in experimental models.

Conclusions

  • Anoikis-associated lncRNAs are significant in endometrial cancer progression and hold potential as prognostic markers and therapeutic targets.
  • The developed prognostic signature may provide new avenues for endometrial cancer treatment and immunotherapy.
  • CFAP58-DT's validated function supports its role in endometrial cancer, warranting further mechanistic investigation.

Keywords:

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