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Updated: Jul 10, 2026
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Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol
Published on: February 21, 2016
11.8K
Kidney Procurement in a Preclinical Large Animal Model.
Eleni M Drivas1, Amanda H Loftin2, Siavash Khaki1
1Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine.
Journal of Visualized Experiments : Jove
|May 19, 2025
Summary
This study details an optimized protocol for swine kidney procurement, crucial for machine perfusion and transplantation research. The standardized technique ensures graft quality and reproducible data in preclinical models.
Area of Science:
- Surgical techniques
- Preclinical research
- Organ transplantation
Background:
- Machine perfusion is vital for ex vivo organ assessment and preservation.
- Large animal models are essential for developing perfusion technologies.
- Standardized organ procurement ensures graft quality and data reproducibility.
Purpose of the Study:
- To describe an optimized protocol for kidney procurement in a preclinical swine model.
- To ensure graft quality and data reproducibility for machine perfusion studies.
Main Methods:
- A midline incision provides access to both kidneys in Yorkshire cross/mix pigs.
- Kidneys are dissected, clamped, resected, and cannulated.
- The technique simulates living or deceased donor settings for transplantation research.
Main Results:
- The protocol allows for efficient kidney recovery in swine.
- The method supports both single and dual kidney procurement scenarios.
- The technique facilitates subsequent autotransplantation or blood collection for machine perfusion.
Conclusions:
- This optimized swine kidney procurement protocol is clinically translatable.
- It provides a standardized method for obtaining high-quality grafts for research.
- The technique is valuable for advancing machine perfusion and transplantation studies.
