Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Drought-responsive TaMIP1 controls root density to confer the tradeoff between grain yield and drought resistance in wheat.

Plant physiology·2026

Same author

TabHLH27 orchestrates root growth and drought tolerance to enhance water use efficiency in wheat.

Journal of integrative plant biology·2024

Same author

QTL Mapping of Trichome Traits and Analysis of Candidate Genes in Leaves of Wheat (<i>Triticum aestivum</i> L.).

Genes·2024

Same author

RING finger E3 ubiquitin ligase gene TaAIRP2-1B controls spike length in wheat.

Journal of experimental botany·2023

Same author

The wheat basic helix-loop-helix gene TabHLH123 positively modulates the formation of crown roots and is associated with plant height and 1000-grain weight under various conditions.

Journal of experimental botany·2023

Same author

Wheat TaSnRK2.10 phosphorylates TaERD15 and TaENO1 and confers drought tolerance when overexpressed in rice.

Plant physiology·2022

Search research articles

Related Experiment Video

Updated: May 21, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

A Raf-Like MAPKKK Gene TaHT1 Controls Drought Tolerance and Primary Root Length in Wheat.

Min Wang¹, Chaonan Li¹, Jingyi Wang¹

¹State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

Plant, Cell & Environment

|May 20, 2025

Summary

A novel wheat gene, high leaf temperature 1 (TaHT1), negatively impacts drought tolerance by interacting with TaSnRK2.10. Its superior allele, Hap-5B-3, offers potential for improving wheat drought resistance in breeding programs.

Keywords:

TaHT1 TaSnRK2.10 drought tolerance functional marker primary root length

More Related Videos

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

Related Experiment Videos

Last Updated: May 21, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

Area of Science:

Plant Molecular Biology
Plant Physiology
Agricultural Science

Background:

Wheat (Triticum aestivum L.) is a vital global food crop susceptible to drought stress.
Mitogen-activated protein kinase kinase kinases (MAPKKKs) are crucial in plant drought signaling, but their specific roles in wheat remain unclear.
Understanding wheat drought tolerance mechanisms is essential for food security.

Purpose of the Study:

To identify and characterize novel MAPKKKs involved in wheat drought tolerance.
To elucidate the molecular mechanism of TaHT1 in regulating drought response.
To explore the potential of TaHT1 alleles for wheat breeding.

Main Methods:

Identification and characterization of a novel wheat MAPKKK, TaHT1.
Analysis of TaHT1 expression under polyethylene glycol (PEG) and abscisic acid (ABA) treatments.
Ectopic expression of TaHT1 in rice to assess its function under drought stress.
Gene expression analysis of drought response and reactive oxygen species (ROS) scavenging pathways.
Sequence polymorphism and association analysis of TaHT1 with root length.

Main Results:

A novel Raf-like MAPKKK, TaHT1, was identified and shown to interact with TaSnRK2.10.
TaHT1 expression is downregulated by PEG and ABA treatments.
Ectopic expression of TaHT1 in rice resulted in drought sensitivity, characterized by increased malondialdehyde (MDA) accumulation and reduced cell membrane stability.
TaHT1 regulates genes in drought response and ROS scavenging pathways.
The TaHT1-5B allele, specifically haplotype Hap-5B-3, is significantly correlated with longer primary root length and has been positively selected during Chinese wheat breeding.

Conclusions:

TaHT1 negatively regulates drought tolerance in wheat through its interaction with TaSnRK2.10.
The superior allele Hap-5B-3 of TaHT1-5B provides a valuable genetic resource for enhancing wheat seedling root development and drought tolerance.
Functional markers for TaHT1 alleles can aid in marker-assisted selection for improved wheat varieties.