Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HME as a transformative platform in pharmaceutics: from molecular dispersion engineering to personalized drug delivery.

International journal of pharmaceutics·2026
Same author

Hot-Melt Extrusion of Bupropion with Three Ethylcellulose Grades for Pellet Feedstock Preparation and Screw-Based 3D Printing of Sustained-Release Tablets.

AAPS PharmSciTech·2026
Same author

3D polyurethane vaginal rings for the delivery of steroid hormones using material extrusion additive manufacturing.

International journal of pharmaceutics·2026
Same author

Preclinical development of a 3D-printed hollow microneedle platform for intradermal delivery of antioxidant-loaded nanocarriers.

International journal of pharmaceutics·2026
Same author

Semi-solid extrusion 3D printing of milk formula-based paediatric chewable dosage forms.

Journal of pharmaceutical sciences·2026
Same author

Preparation of Felodipine-PEG Solid Dispersions by Solvent-Free scCO<sub>2</sub> Processing and Their Translation into Orally Disintegrating Tablets.

ACS omega·2026

Related Experiment Video

Updated: May 22, 2025

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
09:45

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

2.5K

Quality by design (QbD) liposomes engineering using 3D printed Tesla microfluidic arrays.

Kanza Rahali1, Atabak Ghanizadeh Tabriz2, Dennis Douroumis1,2

  • 1Centre for Research Innovation, University of Greenwich, Kent, UK.

Journal of Liposome Research
|May 20, 2025
PubMed
Summary

Quality by Design (QbD) optimizes liposome nanoparticle production using 3D printed Tesla microfluidic arrays. Narrower channels and specific material attributes significantly reduce particle size, outperforming conventional methods.

Keywords:
LiposomesMicrofluidicsNanoparticlesQuality by DesignTesla arrays

More Related Videos

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

8.9K
Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

22.7K

Related Experiment Videos

Last Updated: May 22, 2025

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
09:45

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

2.5K
Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

8.9K
Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

22.7K

Area of Science:

  • Nanotechnology and Materials Science
  • Pharmaceutical Sciences and Drug Delivery

Background:

  • Liposome nanoparticles are crucial for drug delivery systems.
  • Conventional methods like ultrasonication have limitations in controlling nanoparticle characteristics.
  • Microfluidic technology offers precise control over nanoparticle formation.

Purpose of the Study:

  • To apply a Quality by Design (QbD) approach to optimize liposome nanoparticle production.
  • To investigate the impact of 3D printed Tesla microfluidic designs versus ultrasonication.
  • To evaluate critical processing parameters (CPP) and critical material attributes (CMA) on liposome characteristics.

Main Methods:

  • Utilized 3D printed Tesla microfluidic arrays (direct and serpentine shapes) and conventional ultrasonication.
  • Investigated CPPs: Tesla array shape, length, and channel width.
  • Studied CMAs: phosphatidylcholine (PC) carbon chain length and lipid:cholesterol ratio.

Main Results:

  • Liposome size decreased in the order: plain array > Tesla (serpentine) > Tesla (direct) > ultrasonication.
  • Narrow channel width (200 μm) in improved Tesla arrays yielded the smallest liposome size (74 nm).
  • Shorter PC carbon chain length (Lipoid S75) and increased cholesterol content reduced particle size and zeta-potential.

Conclusions:

  • Both CPPs and CMAs critically influence liposome nanoparticle formation and characteristics.
  • 3D printed Tesla microfluidic arrays, particularly with optimized designs, offer superior control over liposome size compared to ultrasonication.
  • QbD approach successfully identified key parameters for reproducible and tunable liposome nanoparticle production.