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Published on: September 5, 2013
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A multilocus sequence typing scheme for the emerging respiratory pathogen Mycoplasma amphoriforme.
Lily Simpson1, Joshua Macleod1, Richard S Rowlands1
1Microbiology and Infection Research Group, Department of Biomedical Sciences, Cardiff Metropolitan University, Cardiff, United Kingdom, CF5 2YB.
Letters in Applied Microbiology
|May 20, 2025
Summary
A new multilocus sequence typing (MLST) scheme for Mycoplasma amphoriforme was developed. This scheme effectively analyzes population structure and transmission dynamics of this emerging respiratory pathogen.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- Mycoplasma amphoriforme is an emerging respiratory pathogen.
- Limited knowledge exists regarding its population structure and transmission dynamics.
Purpose of the Study:
- To develop and validate the first multilocus sequence typing (MLST) scheme for Mycoplasma amphoriforme.
- To apply this MLST scheme to analyze the population structure and transmission of M. amphoriforme.
Main Methods:
- Developed an MLST scheme using four loci (gyrB, atpG, uvrA, rpoB) based on established Mycoplasma MLST schemes.
- Sequenced seven M. amphoriforme isolates from the UK and Denmark for scheme development.
- Applied the MLST scheme to 20 previously sequenced genomes from the UK and France/Tunisia.
Main Results:
- Identified 13 distinct sequence types among 27 M. amphoriforme isolates.
- Generated a phylogenetic tree comparable to whole-genome-based analyses.
- Corroborated potential healthcare-associated transmission between patients.
Conclusions:
- The developed MLST scheme provides a robust tool for analyzing M. amphoriforme population structure.
- The findings support the utility of MLST for understanding transmission dynamics of this emerging pathogen.
- MLST analysis yielded population structure insights comparable to whole-genome studies.
Keywords:
common variable immunodeficiencyemerging pathogenpathogen typingrespiratory tract infectionwhole-genome sequencingMore Related Videos
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