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Identification of oncogenes associated with colorectal cancer mortality and the effect of cinnamon-conjugated magnetic nanoparticles on their expression

  • 0Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.
Scientific Reports +

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May 20, 2025

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May 20, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Iron oxide nanoparticles coated with glucose and cinnamon show potential in treating colorectal cancer (CRC). These nanoparticles reduced cancer cell survival and decreased the expression of key oncogenes, offering a targeted therapy approach.

Area Of Science

  • Nanotechnology
  • Biomedical Engineering
  • Oncology

Background

  • Colorectal cancer (CRC) presents challenges in treatment due to drug resistance and disease severity.
  • Identifying molecular targets and developing targeted therapies are crucial for improving CRC patient outcomes.
  • Iron oxide nanoparticles offer potential for targeted drug delivery due to their magnetic properties.

Purpose Of The Study

  • To synthesize and characterize Fe3O4@Glu-Cinnamon nanoparticles.
  • To investigate the effect of these nanoparticles on colorectal cancer cell survival and apoptosis.
  • To evaluate the impact of the nanoparticles on the expression of specific oncogenes involved in CRC progression.

Main Methods

  • Nanoparticle synthesis and characterization using FT-IR, XRD, DLS, zeta potential, TEM, SEM, EDS-mapping, and VSM.
  • Cytotoxicity assessment via MTT assay.
  • Apoptosis, necrosis, and cell cycle analysis using flow cytometry.
  • Quantitative analysis of oncogene expression (SNAI1, THBS2, INHBA).

Main Results

  • Fe3O4@Glu-Cinnamon nanoparticles were successfully synthesized with spherical morphology and specific physicochemical properties.
  • Nanoparticle treatment significantly increased apoptosis and necrosis in SW480 colorectal cancer cells.
  • Cell cycle arrest was observed in the S and G2/M phases.
  • Expression of oncogenes SNAI1, THBS2, and INHBA was significantly reduced.

Conclusions

  • Fe3O4@Glu-Cinnamon nanoparticles demonstrate significant anti-cancer effects on colorectal cancer cells in vitro.
  • The nanoparticles effectively induce apoptosis and necrosis, and modulate cell cycle progression.
  • Targeted downregulation of key oncogenes suggests a promising therapeutic strategy for colorectal cancer.
  • The magnetic properties of Fe3O4 nanoparticles facilitate potential applications in targeted drug delivery for enhanced efficacy and reduced systemic toxicity.

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