In-vitro anti-inflammatory and antagonistic efficacy of polymyxin B capped silver nanoparticles in multi drug resistant Acinetobacter baumannii

Multidrug resistant Acinetobacter baumannii is declared as crucial level precedence pathogen by World Health Organization that needs new and upgraded antibiotics for better treatment. Against a vast extent of microbes, many silver nanoparticles have displayed anti-microbial activity because of their numerous methods of antimicrobial actions. This study was aimed to isolate and characterize the Acinetobacter baumannii by using standard microbiological technique and to analyze the anti-inflammatory and antagonistic effect of PB capped AgNPs in isolated A. baumannii. In antagonistic activity, PB capped AgNPs showed antagonistic effect in 8 out of 20 isolates tested and PB when combined with AgNPs showed antagonistic effect in all isolates tested. In in-vitro anti-inflammatory egg albumin assay, PB capped AgNPs gave inhibition rate of 96% at 12.5 µg, 93% at 25 µg, 82% at 50 µg and 62% at 100 µg whereas in Polymyxin B combined AgNPs it gave inhibition rate of 97% at the dose of 50 µg. In-silico analysis also showed that PB in combination with tri-sodium citrate gave good binding energy than tri-sodium citrate and Polymyxin used alone. Hence, this study represented that silver nanoparticles when used in combination proved to be a good alternative in the treatment of MDR Acinetobacter baumannii.