Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decreased TXNRD1 is associated with resistance to tagraxofusp in blastic plasmacytoid dendritic cell neoplasms, as seen in phase II.

Leukemia·2026
Same author

Immunophenotypic, Cytogenetic, and Molecular Characterization of NUP98-Rearranged Pediatric Myeloid Neoplasms.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026
Same author

Tumor microbial burden drives immune responses through regulation of Interferon signaling.

Cancer discovery·2026
Same author

All-Oral Combination of Revumenib, Decitabine, and Venetoclax for Relapsed or Refractory AML (SAVE).

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Closing the paediatric gap in cancer drug development: a call to industry.

Nature reviews. Drug discovery·2026
Same author

LATS1/2-CD38 Metabolic Rewiring Links Senescence to Intraplaque Thrombosis.

Circulation research·2026

Related Experiment Video

Updated: May 23, 2025

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
10:18

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

13.7K

Multimodal spatial proteomic profiling in acute myeloid leukemia.

Christopher P Ly1, Ivo Veletic1, Christopher D Pacheco1

  • 1Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

NPJ Precision Oncology
|May 20, 2025
PubMed
Summary

Researchers discovered tertiary lymphoid-like structures in the bone marrow of acute myeloid leukemia (AML) patients. These structures, identified using advanced spatial imaging, may predict patient outcomes and offer new therapeutic targets for AML.

More Related Videos

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
08:59

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

317
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K

Related Experiment Videos

Last Updated: May 23, 2025

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
10:18

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

13.7K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
08:59

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

317
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K

Area of Science:

  • * Hematology and Immunology
  • * Cancer Biology
  • * Spatial Omics

Background:

  • * Acute myeloid leukemia (AML) occurs in an immune-rich bone marrow microenvironment, but immune therapies often lack durable efficacy.
  • * Understanding leukemic interactions within the bone marrow is crucial for improving treatment outcomes.

Purpose of the Study:

  • * To investigate the spatial architecture of the bone marrow microenvironment in AML.
  • * To identify novel structures and cellular interactions influencing immune responses in AML.
  • * To validate findings using multiple omics approaches and assess their predictive value for patient outcomes.

Main Methods:

  • * Development of a high-throughput pipeline for high-plex spatial immunofluorescence and mass cytometry imaging on tissue microarrays.
  • * Unbiased clustering using the spatial K function to identify cellular aggregates.
  • * Validation through spatial transcriptomics, proteomics, and analysis of a public transcriptomic dataset.

Main Results:

  • * Discovery and validation of tertiary lymphoid-like aggregates in the bone marrow of AML patients.
  • * Identification of tertiary lymphoid structure (TLS) signatures predictive of AML patient outcomes.
  • * Demonstration of high-plex spatial proteomics for uncovering novel microenvironmental interactions.

Conclusions:

  • * Tertiary lymphoid-like aggregates are present in the AML bone marrow microenvironment and are associated with patient prognosis.
  • * Advanced spatial omics techniques provide powerful tools for dissecting complex tissue microenvironments.
  • * The developed methodologies are adaptable for studying other bone marrow diseases facing decalcification and autofluorescence challenges.