Proteomics and succinylation modification characterization in clear cell renal cell carcinoma

Area of Science:

• Biochemistry
• Oncology
• Proteomics

Background:

• Clear cell renal cell carcinoma (ccRCC) is an aggressive kidney cancer with high mortality.
• Abnormal protein succinylation is implicated in various diseases, including cancer, but its role in ccRCC is unstudied.
• Understanding succinylation in ccRCC is crucial for developing novel therapeutic targets.

Purpose of the Study:

• To comprehensively analyze the global proteome and succinylation modification landscape in ccRCC.
• To identify differentially succinylated proteins and pathways involved in ccRCC progression.
• To explore potential therapeutic strategies targeting aberrant succinylation in ccRCC.

Main Methods:

• Quantitative proteomics and succinylation modification omics were employed.
• High-throughput liquid chromatography-mass spectrometry was used for analysis.
• ccRCC tissues and adjacent non-cancerous tissues were compared.

Main Results:

• Identified 4801 lysine succinylation sites across 1274 proteins.
• Found 434 differentially succinylated sites in 328 proteins in ccRCC.
• Differentially succinylated proteins are mainly involved in mitochondrial energy metabolism pathways.

Conclusions:

• This is the first study to provide a global succinylation modification profile in ccRCC.
• Aberrant succinylation, particularly in energy metabolism, is linked to ccRCC progression.
• Reversing abnormal succinylation presents a potential new therapeutic avenue for ccRCC.