Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

1.3K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IglF mediates type VI secretion system spike assembly and promotes <i>Francisella</i> virulence.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Outer Membrane Vesicles Mediate the Secretion and Nuclear Trafficking of a Bacterial Nucleomodulin.

Journal of extracellular vesicles·2026
Same author

Differential roles of the type I and II secretion systems for the intracellular ABC141 Acinetobacter baumannii infection, which elicits an atypical hypoxia response in endothelial cells.

PLoS pathogens·2026
Same author

The <i>Brucella</i> type IV secretion system and effector proteins.

microLife·2025
Same author

Functional and intricate interaction network connecting <i>Helicobacter pylori</i> Cag type 4 secretion system surface proteins with outer membrane proteins HopQ and HopZ.

microLife·2025
Same author

Widespread deployment of the human CD38 ADP-ribosyl cyclase fold in antibacterial and anti-eukaryotic polymorphic toxins.

The Journal of biological chemistry·2025

Related Experiment Video

Updated: May 22, 2025

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
15:29

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

8.2K

Brucella NyxA and NyxB dimerization enhances effector function during infection.

Lison Cancade-Veyre1,2, Arthur Louche1, Francine C A Gérard1

  • 1Laboratory of Molecular Microbiology and Structural Biochemistry, Centre National de la Recherche Scientifique UMR5086, Université de Lyon, France.

FEBS Letters
|May 21, 2025
PubMed
Summary

Brucella abortus infection relies on NyxA and NyxB effectors. Their dimerization is crucial for function, enabling better delocalization of the host target sentrin-specific protease 3 (SENP3) during pathogen invasion.

Keywords:
Brucella abortusNyxdimerizationeffectorspathogenesis

More Related Videos

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
10:39

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Published on: November 27, 2014

8.2K
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
12:54

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Published on: May 19, 2023

1.7K

Related Experiment Videos

Last Updated: May 22, 2025

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
15:29

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

8.2K
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
10:39

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Published on: November 27, 2014

8.2K
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
12:54

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Published on: May 19, 2023

1.7K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Pathogenesis

Background:

  • Brucella abortus causes widespread zoonotic diseases.
  • The translocated effectors NyxA and NyxB are involved in Brucella infection.
  • The functional significance of NyxA and NyxB interactions and dimerization was previously unknown.

Purpose of the Study:

  • To investigate the interaction between NyxA and NyxB.
  • To determine the role of NyxA and NyxB dimerization in Brucella pathogenesis.
  • To elucidate the mechanism by which these effectors modulate host targets.

Main Methods:

  • Co-immunoprecipitation assays to study protein interactions.
  • Bacterial infection models to assess effector function in vivo.
  • Cellular localization studies to track host target delocalization.

Main Results:

  • NyxA and NyxB directly interact, forming dimers.
  • Dimerization of NyxA and NyxB is essential for their function during Brucella infection.
  • Monomeric Nyx effectors bind to sentrin-specific protease 3 (SENP3) but are less effective at delocalizing it from nucleoli compared to dimers.

Conclusions:

  • NyxA and NyxB dimerization is a key molecular mechanism in Brucella pathogenesis.
  • Understanding these intra- and inter-effector interactions provides novel insights into pathogen strategies.
  • Targeting effector dimerization could represent a future therapeutic approach against Brucella infections.