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Development and Application of the Modular MasSpec Pen System for Clinical Opioid Screening.

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Area of Science:

  • Analytical Chemistry
  • Forensic Science
  • Biomedical Engineering

Background:

  • Mass spectrometry (MS) offers high sensitivity and specificity for small molecule analysis, crucial for opioid screening.
  • Direct detection methods are sought to streamline patient screening workflows.
  • Current methods may require complex sample preparation or lack directness.

Purpose of the Study:

  • To develop and apply a modular MasSpec Pen (MSPen) system for opioid screening.
  • To decouple sampling and MS analysis for flexible and rapid opioid detection.
  • To evaluate the MSPen system's performance for direct opioid analysis from human skin.

Main Methods:

  • Development of a modular MSPen system separating sampling and MS analysis.
  • Utilized electrospray ionization MS for detecting various opioids (oxycodone, hydrocodone, fentanyl, hydromorphone, morphine).
  • Analyzed opioid standards and doped human skin, followed by clinical testing on patient skin, urine, and saliva.

Main Results:

  • Achieved detection and quantification limits in the single nanogram range for opioids on surfaces and ex vivo skin.
  • Demonstrated feasibility of the modular MSPen system for direct opioid sampling from patient skin.
  • Showcased semi-quantitation of opioids directly from skin surfaces, comparable to urine and saliva tests.

Conclusions:

  • The modular MSPen system enables direct, non-destructive sampling and semi-quantitation of opioids from surfaces and skin.
  • This technology offers a simplified and expedited approach to opioid screening.
  • The MSPen system holds potential for broader applications in drug screening and diagnostics.