Related Experiment Video
Updated: May 23, 2025

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
Published on: November 2, 2018
Mechanotransduction in distinct F-actin architectures: a novel molecular tension sensor revealing cellular mechanical
Ting Liang1, Bin Li1
1Medical 3D Printing Center, Orthopedic Institute, Department of Orthopedic Surgery, The First Affiliated Hospital, School of Biology and Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215000, PR China.
Abstract:
Mechanotransduction is essential for cell fate and behavior, and F-actin plays a key role in the generation and transmission of molecular forces. A recent study published in Nature Communication presented a novel high-precision molecular tension measurement method using a Förster resonance energy transfer-based tension sensor with separated load-bearing function within distinct F-actin structures, and demonstrated that cellular mechanical anisotropy depends on cell shape, loading direction, and magnitude.
More Related Videos
09:43A Protocol for Using Förster Resonance Energy Transfer (FRET)-force Biosensors to Measure Mechanical Forces across the Nuclear LINC Complex
Published on: April 11, 2017
07:20Imaging Integrin Tension and Cellular Force at Submicron Resolution with an Integrative Tension Sensor
Published on: April 25, 2019
Related Concept Videos
Tension Response at Adherens Junctions
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...
Studying the Cytoskeleton
Generation of Straight or Branched Actin Filaments
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Mechanical Protein Functions