Related Experiment Video

Updated: May 23, 2025

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Published on: May 25, 2015

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MicroRNA let-7f protects against H2O2-induced oxidative damage in neuroblastoma cells by targeting AKT-2

Kuo Li1,2,3, Zhan-Qiang Wang1,2,4, Jun-Ling Zhang3

  • 1Department of Neurology, Hebei Medical University, Shijiazhuang, Hebei, China.

Archives of Medical Science : AMS
|May 21, 2025
PubMed
Abstract

Insights

MicroRNA let-7f protects against oxidative damage in Alzheimer's disease models. Upregulating miR-let-7f in cells reduced damage by targeting AKT-2, offering new insights into AD pathogenesis.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Alzheimer's disease (AD) is a leading cause of dementia.
  • MicroRNAs (miRNAs) are implicated in AD pathogenesis.
  • The role of miR-let-7f in oxidative damage in AD requires elucidation.

Purpose of the Study:

  • To investigate the role of miR-let-7f in oxidative damage in SH-SY5Y cells.
  • To determine if miR-let-7f affects apoptosis and cell viability under oxidative stress.
  • To identify the direct target gene of miR-let-7f.

Main Methods:

  • miRNA microarray analysis to detect differential expression in AD patients.
  • Cell viability and apoptosis assays to evaluate cell injury.
  • Quantitative real-time PCR (qRT-PCR) and Western blot to measure gene and protein expression.
  • Luciferase reporter gene assay to identify miRNA targets.

Main Results:

  • miR-let-7f was overexpressed in AD patients.
  • Hydrogen peroxide (H2O2) induced apoptosis in SH-SY5Y cells, with concurrent upregulation of miR-let-7f.
  • Upregulation of miR-let-7f enhanced cell viability and inhibited apoptosis under H2O2 exposure.
  • AKT-2 was identified as a direct target of miR-let-7f, with miR-let-7f negatively regulating AKT-2 expression.

Conclusions:

  • Upregulation of miR-let-7f alleviates H2O2-induced oxidative damage in SH-SY5Y cells by targeting AKT-2.
  • These findings offer a novel perspective on the role of miR-let-7f in AD pathogenesis.
  • miR-let-7f represents a potential therapeutic target for mitigating oxidative damage in Alzheimer's disease.
Keywords:
AKT-2Alzheimer’s diseasemiR-let-7fmicroRNAoxidative damage

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