Related Experiment Video

Updated: May 23, 2025

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.4K

Long noncoding RNA MALAT1 can regulate proliferation and apoptosis of LPS-treated HK-2 cells via targeting miR-23a-3p

Jinxia Li1, Haibo Liu2, Chao Li2

  • 1Department of Critical Care, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.

Archives of Medical Science : AMS
|May 21, 2025
PubMed
Abstract

Insights

Long noncoding RNA MALAT1 is elevated in sepsis-induced acute kidney injury. MALAT1 regulates cell proliferation and apoptosis via the miR-23a-3p and ERK signaling pathway, offering a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Renal Physiology

Background:

  • Sepsis-induced acute kidney injury (septic AKI) is a critical condition with high mortality.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in various diseases, including AKI.

Purpose of the Study:

  • To investigate the role of lncRNA MALAT1 in septic AKI.
  • To elucidate the underlying molecular mechanism involving miR-23a-3p and ERK signaling.

Main Methods:

  • Serum samples from septic AKI patients and healthy controls were analyzed for MALAT1 levels.
  • In vitro experiments using LPS-treated HK-2 cells assessed the effects of MALAT1 and miR-23a-3p on cell proliferation and apoptosis.
  • The involvement of ERK signaling was investigated.

Main Results:

  • MALAT1 levels were significantly increased in septic AKI patients and LPS-treated cells.
  • Overexpression of MALAT1 mitigated LPS-induced kidney cell injury.
  • MALAT1 was confirmed to target miR-23a-3p, and MALAT1 siRNA promoted cell proliferation and inhibited apoptosis by activating ERK signaling.
  • Knockdown of miR-23a-3p partially reversed the anti-apoptotic effects of MALAT1 siRNA.

Conclusions:

  • The MALAT1/miR-23a-3p axis plays a crucial role in regulating proliferation and apoptosis in septic AKI.
  • This axis modulates ERK signaling, suggesting it as a potential therapeutic target for septic AKI.
Keywords:
MALAT1acute kidney injurymiR-23a-3psepsis

More Related Videos

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury08:27

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Published on: May 21, 2018

10.8K
Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

940

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.1K