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Updated: May 23, 2025

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Published on: July 21, 2017
The role of autophagy in the Arabidopsis self-incompatible pollen rejection response
Stuart R Macgregor1, Daphne R Goring1
1Department of Cell & Systems Biology, University of Toronto, Toronto, Canada M5S 3B2.
Abstract:
In plants, macroautophagy/autophagy is an essential mechanism responsible for a large variety of processes throughout the plant's lifecycle, including nutrient processing, immunity, stress responses and senescence. Previous studies had observed the presence of autophagosomes in an Arabidopsis sexual reproduction system that prevents self-fertilization (self-incompatibility), but their requirement in this pathway was unclear. Using autophagy-deficient mutants, we have recently found that autophagy is a key contributor in the Arabidopsis self-incompatibility response to reject self-pollen.
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