Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AFMap-UNet enables accurate nuclear segmentation of atomic force microscopy images with minimal training data.

Scientific reports·2026
Same author

The global landscape of SCD and approach to case work-up in Latin America: a LAHRS and SOBRAC survey.

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·2026
Same author

Ancestry-informative regulatory variants at KCNB1 modulate adipogenesis and body mass index.

Frontiers in endocrinology·2026
Same author

Danon Disease: Understanding the Role of LAMP2 Variants in Cardiomyopathy and Multisystemic Involvement.

Arquivos brasileiros de cardiologia·2026
Same author

A deep learning ECG model for identification and localization of occlusion myocardial infarction.

Nature communications·2026
Same author

Discovery of gene-alcohol interaction loci influencing blood pressure in 1.1 million individuals from multiple populations.

Research square·2026

Related Experiment Video

Updated: May 23, 2025

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.3K

Exploring focal adhesion data: dynamic parameter extraction from FRAP and FLAP experiments using chemical master

Luciana Renata de Oliveira1, Matheus Gimenez Fernandes1, José Salvatore Leister Patane1

  • 1Laboratório de Genetica e Cardiologia Molecular, Instituto do Coração (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil.

Frontiers in Molecular Biosciences
|May 21, 2025
PubMed
Summary

This study introduces a new stochastic model for analyzing protein dynamics using FRAP and FLAP experiments. The model enhances understanding of molecular interactions and protein turnover in cellular networks.

Keywords:
FLAPFRAPchemical master equationfocal adhesionprotein dynamicsprotein interaction

More Related Videos

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

14.9K
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
08:28

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Published on: November 2, 2018

8.2K

Related Experiment Videos

Last Updated: May 23, 2025

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.3K
Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

14.9K
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
08:28

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Published on: November 2, 2018

8.2K

Area of Science:

  • Cell Biology
  • Biophysics
  • Systems Biology

Background:

  • Fluorescence recovery after photobleaching (FRAP) and fluorescence loss after photobleaching (FLAP) are key techniques for studying protein dynamics.
  • Traditional models often oversimplify molecular interactions, failing to capture stochastic effects.
  • Focal adhesions (FAs) are crucial cellular structures where protein dynamics play vital roles in mechanotransduction and cytoskeletal organization.

Purpose of the Study:

  • To develop a novel stochastic model for analyzing FRAP and FLAP data.
  • To extract detailed dynamic parameters, including protein entry and exit rates, beyond standard analyses.
  • To investigate protein turnover and interactions within the focal adhesion network.

Main Methods:

  • Developed a stochastic model based on the analytical solution of the chemical master equation.
  • Applied the model to analyze existing FRAP/FLAP experimental data from GFP-tagged focal adhesion proteins in NIH3T3 fibroblasts.
  • Constructed a protein interaction network to assess the influence of vinculin and actin on talin dynamics.

Main Results:

  • The novel stochastic model successfully extracted dynamic parameters from FRAP and FLAP data.
  • Identified distinct dynamic behaviors for various focal adhesion proteins involved in mechanotransduction and adhesion.
  • Uncovered regulatory roles of vinculin and actin in talin dynamics and focal adhesion turnover.

Conclusions:

  • The developed stochastic model offers a generalizable framework for analyzing protein dynamics from FRAP and FLAP data.
  • This approach provides deeper insights into molecular interactions and cellular processes, applicable to various biological systems.
  • The model can be used for new experimental data and reanalysis of existing datasets to reveal hidden molecular mechanisms.