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Related Concept Videos

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Application of CRISPR-Based Epigenome Editing Tools for Engineering Programmable Embryo Models.

Gerrald A Lodewijk1,2,3, Sayaka Kozuki1,2,3, Carly Guiltinan1,2,3

  • 1Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 21, 2025
PubMed
Summary
This summary is machine-generated.

This study details a method using CRISPR activation to control cell fate in embryonic stem cells, crucial for building better stem cell-based embryo models for developmental biology research.

Keywords:
CRISPR activationEmbryo modelsEmbryoidsEpigenomeMammalian embryoPluripotent stem cells

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Epigenetics

Background:

  • Stem cell-based embryo models (SEMs) are vital for studying early human embryogenesis.
  • Generating key extraembryonic lineages (trophoblast and hypoblast) is essential for SEMs.
  • These lineages support embryonic development and provide critical signaling cues.

Purpose of the Study:

  • To present a method for controlling cell fate in embryonic stem cells.
  • To engineer improved stem cell-based embryo models.
  • To leverage CRISPR epigenome editing for developmental biology research.

Main Methods:

  • Utilized CRISPR activation (CRISPRa), a technology for precise gene activation.
  • Developed a step-by-step protocol for implementing CRISPRa in embryonic stem cells.
  • Applied the method to generate CRISPR-programmed mouse embryo models.

Main Results:

  • Demonstrated efficient and precise activation of cell fate-determining factors using CRISPRa.
  • Successfully controlled cell fate in embryonic stem cells.
  • Established a foundation for engineering advanced SEMs.

Conclusions:

  • CRISPRa offers a powerful tool for manipulating cell fate in stem cells.
  • This method advances the development of sophisticated stem cell-based embryo models.
  • The findings have implications for understanding early embryonic development and lineage specification.