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Indolylamide Macrocyclization by a Streptococcus pneumoniae ThiF-like Enzyme Family Member
Anshul Rajput1, Keelie S Butler1, Daniel A Springer1
1Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, Greensboro, North Carolina 27412, United States.
Abstract:
ThiF-like enzymes are present in diverse RiPP biosynthetic pathways and are known to catalyze reactions such as thiolactone and phosphoramidate bond formation. To uncover new chemical space for ThiF-like enzymes, we utilized a global genome mining approach and identified a minimal ind RiPP cluster in the human pathogen Streptococcus pneumoniae. In vitro characterization of IndF demonstrated the first indolylamide (Trp-Ile) linkage in a RiPP pathway and a new reaction type catalyzed by a ThiF-like enzyme.
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