Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial transcriptome profiling links SERPINA3 to nerve-associated immunosuppression in pancreatic cancer.

Biochemical and biophysical research communications·2026
Same author

Efficacy of gut-brain neuromodulators in functional esophageal disorders: a systematic review.

BMC gastroenterology·2026
Same author

Tunable and Selective Doping Modulation in Pd-Filled Carbon Nanotube Transistors.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Experiences of Newborn Fathers in Supporting Breastfeeding Based on the Social-Ecological System: A Qualitative Study.

Journal of nursing management·2026
Same author

Influence of Mix Composition on the Microstructural Evolution of Leached Cement Pastes.

Materials (Basel, Switzerland)·2026
Same author

Predictive efficacy of PASP combined with NT-proBNP for outcomes in pregnant women with severe cardiovascular disease: a single-centre retrospective observational study.

BMC pregnancy and childbirth·2026

Related Experiment Video

Updated: Jun 13, 2025

Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps
10:55

Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps

Published on: January 31, 2025

316

Protocol for measuring the Young's modulus of organoids using atomic force microscopy.

Tianzhen Zhang1, Lina Ma1, Shen Ling1

  • 1Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; State Key Lab of Digestive Health, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; National Clinical Research Center for Digestive Diseases, Beijing 100050, China.

STAR Protocols
|May 21, 2025
PubMed
Summary

This study introduces a standardized atomic force microscopy (AFM) protocol for measuring organoid stiffness. The new method enhances reproducibility in biomechanical research by optimizing probe use and force-curve analysis.

Keywords:
AFMAtomic force microscopyBiophysicsCancerOrganoids

More Related Videos

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
08:41

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

40.0K
Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
11:18

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues

Published on: July 15, 2019

11.4K

Related Experiment Videos

Last Updated: Jun 13, 2025

Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps
10:55

Extracting the Young's Modulus of Native Murine Pulmonary Basement Membranes from Atomic Force Microscopy Derived Force Maps

Published on: January 31, 2025

316
Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
08:41

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Published on: June 27, 2013

40.0K
Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
11:18

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues

Published on: July 15, 2019

11.4K

Area of Science:

  • Biotechnology
  • Biophysics
  • Materials Science

Background:

  • Atomic force microscopy (AFM) is widely used for cell and tissue mechanics.
  • A standardized method for assessing organoid stiffness using AFM is currently lacking.
  • Accurate mechanical characterization of organoids is crucial for understanding their development and function.

Purpose of the Study:

  • To present a standardized protocol for quantifying the Young's modulus of organoids using AFM.
  • To improve the reproducibility and reliability of organoid stiffness measurements.
  • To expand the capabilities of biomechanical research involving organoids.

Main Methods:

  • Developed a protocol combining force-curve analysis with an optimized AFM probe.
  • Detailed steps for organoid preparation, OCT embedding, and slicing.
  • Included procedures for AFM detection and comprehensive force-curve analysis.

Main Results:

  • The protocol enables precise quantification of organoid Young's modulus.
  • Mechanically addressing gaps within organoids enhances measurement reproducibility.
  • The optimized approach provides reliable biomechanical data for organoids.

Conclusions:

  • The presented AFM protocol offers a standardized approach for organoid stiffness assessment.
  • This method improves the accuracy and reproducibility of biomechanical measurements in organoid research.
  • The protocol facilitates advanced biomechanical studies and applications using organoids.