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Updated: Jun 13, 2025
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
Published on: August 11, 2017
Protocol for embedded 3D bioprinting of arterial models with customized contractile and metabolic functions
Huilong Du1, Shuyuan Yu2, Ziqi Gao2
1School of Engineering, Hangzhou Normal University, Hangzhou 311121, People's Republic of China; State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou 310058, People's Republic of China; School of Mechanical Engineering, Zhejiang University, Hangzhou 310058, People's Republic of China.
Abstract:
3D bioprinting enables the fabrication of complex tissue structures in vitro. Here, we present a protocol for constructing multilayered arterial tissues with cellular alignment that enhances vascular smooth muscle function by modulating contractile and synthetic pathways. We describe steps for material preparation, simulation, embedded bioprinting, and biological characterization. This approach is adaptable to other tissues with cellular alignment, including skeletal and cardiac muscles. For complete details on the use and execution of this protocol, please refer to Li et al.1.
