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Investigating enhanced stability in CTAB(C)-compacted DNA under aging conditions for data storage.

Anshula Tandon1, Yeonju Nam1, Seongjun Seo1

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Summary
This summary is machine-generated.

DNA compaction using surfactants and decompaction with cyclodextrins effectively preserves both natural and synthetic DNA during accelerated aging. This method enhances DNA stability for long-term data storage applications.

Keywords:
DNAaccelerated agingcationic surfactantscompaction and decompactiondata storage

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Area of Science:

  • Biotechnology
  • Materials Science
  • Genomics

Background:

  • DNA offers high information density and longevity for data storage.
  • Preserving DNA integrity over extended periods remains a significant challenge.
  • Surfactant-mediated compaction and cyclodextrin-driven decompaction are explored for DNA stabilization.

Purpose of the Study:

  • To evaluate the effectiveness of surfactant-mediated compaction and cyclodextrin-driven decompaction for long-term DNA storage.
  • To assess the stability of compacted natural (salmon-derived DNA) and synthetic DNA under accelerated aging conditions.
  • To determine the feasibility of recovering and sequencing stored DNA for data retrieval.

Main Methods:

  • Compaction of salmon-derived DNA (sDNA) and synthetic DNA (synDNA) using cetyltrimethylammonium bromide (CTAB) and cetyltrimethylammonium chloride (CTAC).
  • Accelerated aging of compacted DNA at temperatures ranging from 4 °C to 70 °C and 50% relative humidity for 4, 8, and 12 days.
  • Decompaction using 2-hydroxypropyl-β-cyclodextrin (2HP-β-CD) followed by analysis using absorbance measurements, quantitative PCR, and Sanger sequencing.

Main Results:

  • Successful recovery and sequencing of both sDNA and synDNA after accelerated aging.
  • Compacted synDNA demonstrated enhanced stability compared to pristine synDNA, especially at 60 °C.
  • Recovered synDNA showed high sequence identities, confirming information preservation.
  • The half-lives of decompacted aged-synDNA at elevated temperatures were comparable to DNA stored in silica matrices.

Conclusions:

  • Surfactant-mediated compaction and cyclodextrin-driven decompaction provide an effective strategy for long-term DNA data storage.
  • The method offers advantages in simplicity and recovery rates compared to existing techniques.
  • This approach shows significant potential for practical DNA-based data storage applications, ensuring data integrity and accessibility.