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Comparative susceptibility of embryonated hen eggs and pig lung alveolar macrophages to chlamydial infection
Veterinary Research Communications
|April 1, 1985
Abstract:
Pig lung alveolar macrophages provided a suitable cell culture system for primary isolation of ovine chlamydia and sustained their growth through several passages. In centrifuged preparations, titres of established strains compared favourably with those obtained in fertile eggs, and in parallel titrations on primary isolation, evidence of chlamydial infection was detectable in macrophages several days earlier than in eggs.
Insights
Pig lung alveolar macrophages offer a superior system for isolating and growing ovine chlamydia. This cell culture method detects chlamydial infections earlier than traditional fertile egg methods.
Area of Science:
- Veterinary Microbiology
- Cell Biology
Background:
- Chlamydia are obligate intracellular bacteria that cause significant disease in livestock.
- Current methods for isolating and propagating Chlamydia, such as using fertile eggs, can be time-consuming and less sensitive.
Purpose of the Study:
- To evaluate pig lung alveolar macrophages as a primary cell culture system for the isolation and propagation of ovine Chlamydia.
- To compare the efficiency and sensitivity of this cell culture system with traditional methods.
Main Methods:
- Primary isolation and serial passage of ovine Chlamydia strains in pig lung alveolar macrophages.
- Titration of Chlamydia in macrophage cultures and fertile eggs.
- Comparison of Chlamydia titres and detection times between the two systems.
Main Results:
- Pig lung alveolar macrophages supported the primary isolation and sustained growth of ovine Chlamydia through multiple passages.
- Established Chlamydia strains in macrophages showed comparable titres to those in fertile eggs.
- Chlamydial infection was detected several days earlier in macrophages than in fertile eggs during primary isolation.
Conclusions:
- Pig lung alveolar macrophages represent a viable and sensitive alternative cell culture system for the diagnosis and study of ovine Chlamydia.
- This method offers potential advantages in early detection and propagation of Chlamydia compared to fertile eggs.