Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
DNA Isolation01:24

DNA Isolation

37.5K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
37.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association Between Brachytherapy and Acute Diarrhea During Chemoradiotherapy for Cervical Cancer: A Multicenter Retrospective Study.

Anticancer research·2026
Same author

Preventive effect of heat-killed Lactiplantibacillus plantarum FM8 in an ovalbumin-induced food allergy murine model.

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology·2026
Same author

Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma development.

Nature communications·2026
Same author

Versatile electroporation protocols enable reproducible CRISPR-RNP delivery across multiple primary mouse cells of the hematopoietic lineage.

Frontiers in immunology·2026
Same author

Regulatory plasticity balances photosynthetic electron flow with enhanced pH-dependent cytochrome b6f control.

Plant physiology·2026
Same author

Development of an inexpensive, simple purification method for ω-5 gliadin from wheat flour.

MethodsX·2026
Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026
Same journal

Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.

Nature biotechnology·2026
Same journal

A retargeted recombinase for precise insertion of large DNA.

Nature biotechnology·2026
Same journal

Experiment-guided AlphaFold3 resolves measurement-consistent protein ensembles.

Nature biotechnology·2026
Same journal

Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026
Same journal

Mapping the spatial landscape of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026
See all related articles

Related Experiment Video

Updated: May 23, 2025

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.0K

A multi-kingdom genetic barcoding system for precise clone isolation.

Soh Ishiguro1, Kana Ishida2, Rina C Sakata1

  • 1School of Biomedical Engineering, Faculty of Applied Science and Faculty of Medicine, The University of British Columbia, Vancouver, British Columbia, Canada.

Nature Biotechnology
|May 21, 2025
PubMed
Summary
This summary is machine-generated.

CloneSelect uses DNA barcodes and CRISPR base editing to isolate specific cell clones from complex populations. This genetic barcoding system enables targeted cell isolation for various research applications.

More Related Videos

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

10.5K
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.2K

Related Experiment Videos

Last Updated: May 23, 2025

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.0K
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

10.5K
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • DNA barcoding facilitates analysis of cell clone dynamics and transcriptomics.
  • Isolating specific phenotypes from heterogeneous cell populations is a significant challenge.

Purpose of the Study:

  • To develop a versatile genetic barcoding system for isolating target cell clones based on phenotype.
  • To enable targeted reporter gene expression for clone isolation using CRISPR base editing.

Main Methods:

  • Developed CloneSelect, a multi-kingdom genetic barcoding system.
  • Utilized barcode-specific CRISPR base editing to trigger reporter gene expression in target clones.
  • Integrated with single-cell RNA sequencing for comprehensive analysis.

Main Results:

  • Demonstrated successful isolation of target clones in diverse cell types including human and mouse cells, yeast, and bacteria.
  • CloneSelect proved scalable and compatible with downstream analyses like single-cell RNA sequencing.
  • Enabled isolation of clones with specific phenotypes from complex mixtures.

Conclusions:

  • CloneSelect offers a powerful and versatile method for isolating specific cell clones across multiple kingdoms.
  • This technology advances the study of cellular heterogeneity and phenotype-driven research.
  • Facilitates precise manipulation and analysis of cell populations in various biological systems.