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Published on: August 7, 2017
Correlation between preoperative and postoperative miR-29c-3p level changes and immune response in paediatric acute
Li Hong1, Baohui Yin2, Chunsheng Duan3
1Department of Laboratory, Haikou Hospital of The Maternal and Child Health, Haikou, 570102, China.
Insights
MicroRNA-29c-3p (miR-29c-3p) levels are elevated in children with acute suppurative appendicitis (ASA) and correlate with postoperative complications. Monitoring miR-29c-3p may help predict adverse outcomes in pediatric ASA patients.
Area of Science:
- Pediatric Surgery
- Molecular Diagnostics
- Inflammation Research
Background:
- Acute suppurative appendicitis (ASA) in children poses significant risks of severe postoperative complications.
- Understanding biomarkers for predicting these complications is crucial for effective patient management.
Purpose of the Study:
- To investigate the expression of miR-29c-3p in pediatric ASA patients.
- To assess the correlation between miR-29c-3p levels and postoperative complications.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to measure miR-29c-3p expression.
- Routine blood tests and ELISA were employed to quantify immune cells, CRP, and inflammatory factors (TNF-α, IL-6).
- A retrospective analysis included 128 children with ASA and 93 healthy controls.
Main Results:
- Preoperative miR-29c-3p levels were higher in ASA patients compared to controls.
- Postoperative miR-29c-3p levels decreased significantly after surgery in ASA patients.
- Elevated miR-29c-3p expression correlated positively with immune markers and a higher incidence of postoperative complications.
Conclusions:
- miR-29c-3p expression is linked to immune responses and inflammatory markers in pediatric ASA.
- miR-29c-3p serves as a potential predictive biomarker for postoperative complications in children with ASA.
Background:
Postoperative complications in children with acute suppurative appendicitis (ASA) can lead to very serious consequences. In this study, we investigated the pre- and postoperative expression of miR-29c-3p in ASA and its value in predicting postoperative complications.
Methods:
Retrospectively, 128 children with ASA and 93 healthy children were included. The qRT-PCR technique was used to detect miR-29c-3p expression in children with ASA preoperatively and 24 h postoperatively. The levels of immune cells (WBC, NEU, LYM) were detected by routine blood test, and the levels of immune proteins CRP and inflammatory factors (TNF-α and IL-6) were detected by ELISA.
Results:
Before surgery, children with ASA had higher levels of miR-29c-3p than healthy controls. But after surgery, miR-29ac-3p levels dropped a lot in children with ASA. The serum levels of immune cells (WBC, NEU, LYM), immune protein CRP and inflammatory factors (TNF-α and IL-6) were significantly decreased in children with ASA. Postoperative miR-29c-3p levels were positively correlated with the levels of immune indicators. The patients with higher miR-29c-3p expression levels showed a greater incidence of postoperative complications.
Conclusions:
The expression of miR-29c-3p was positively correlated to immune cells, immune proteins, and inflammatory factors. The expression levels of miR-29c-3p were related to the occurrence of postoperative complications and could, therefore, be utilized to predict the occurrence of postoperative complications in children with ASA.
