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Updated: Apr 28, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
Published on: November 13, 2017
Exploring the Cancer Immune Epigenome by ATAC-Seq
Chunling Zeng1, Yuexiang Wang2
1CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Abstract:
The Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) is a rapid and sensitive method for exploring the cancer immune epigenome. ATAC-seq captures open chromatin sites based on transposing the sequencing adapter directly into native chromatin in vitro. Here we describe an optimized protocol for ATAC-seq with four main steps: nucleus extraction, transposition, library amplification, sequencing and data analysis. The ATAC protocol has the advantages of low sample requirements, high speed, and applicability to a wide range of cell and tissue types.
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