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Related Experiment Video

Updated: Sep 20, 2025

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes.

Elisabetta Furlanis¹, Min Dai¹, Brenda Leyva Garcia¹

¹Harvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

|May 22, 2025

Summary

Researchers developed novel viral tools using enhancers to precisely target specific neuronal subtypes in the brain. These enhancer-AAV tools enable detailed study of neural circuits and offer potential for targeted therapies.

Keywords:

GABAergic adeno-associated virus cholinergic cortex enhancer interneuron

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Subtype-selective Electroporation of Cortical Interneurons

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

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Last Updated: Sep 20, 2025

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

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Published on: March 31, 2022

Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus

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Published on: September 9, 2020

Subtype-selective Electroporation of Cortical Interneurons

Subtype-selective Electroporation of Cortical Interneurons

Published on: August 18, 2014

Area of Science:

Neuroscience
Molecular Biology
Genomics

Background:

Specific viral tools for targeting neuronal subtypes are limited.
Enhancers incorporated into adeno-associated virus (AAV) vectors can restrict transgene expression to specific neuronal populations.

Purpose of the Study:

To systematically identify enhancer candidates for telencephalic interneuron subtypes using single-cell genomic data.
To establish and validate novel enhancer-AAV tools for precise targeting of distinct neuronal populations.

Main Methods:

Systematic analysis of single-cell genomic data.
Development and testing of enhancer-AAV vectors.
Validation of tools across species.

Main Results:

Identified novel enhancer candidates for telencephalic interneuron subtypes.
Established highly specific enhancer-AAV tools for cortical interneurons and striatal cholinergic interneurons.
Demonstrated the utility of these tools for targeting, observing, and manipulating specific neuronal subtypes.

Conclusions:

Provided a powerful set of enhancer-AAV tools for neuroscience research.
These tools enable precise study of neural circuits and functions.
Offers potential for developing targeted therapies for neurological disorders.