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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

Updated: Sep 20, 2025

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Sorting high-affinity aptamers in a single selection round

Gayathri Kasirajan^1,2, William H Thiel^3,4,5

¹Carver College of Medicine, University of Iowa, Iowa City, IA, USA.

Nature Chemical Biology

|May 22, 2025

Summary

No abstract available in PubMed .

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