Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Destabilization of Microtubules01:45

Destabilization of Microtubules

2.6K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.6K
Telomeres and Telomerase02:41

Telomeres and Telomerase

23.2K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
23.2K
Microtubule Instability02:17

Microtubule Instability

5.0K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.0K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.0K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.5K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.5K
DNA Topoisomerases02:02

DNA Topoisomerases

31.1K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
31.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shadow Hamiltonian in molecular dynamics simulations: Against a possible suggested misuse of its physical meaning.

The Journal of chemical physics·2026
Same author

Unraveling G-Quadruplex and i-Motif Coexistence Within a Double-Stranded DNA.

Angewandte Chemie (International ed. in English)·2026
Same author

Phosphate anions-induced liquid-liquid phase separation in silk fibroin systems to produce biologically stable patches.

International journal of biological macromolecules·2026
Same author

Ligands reshape the compactness, stability, and topology of telomeric G-quadruplex dimers.

Nucleic acids research·2026
Same author

Hard meets soft: tuning binary ferrofluids.

Nanoscale·2026
Same author

Sodium Dodecylbenzene Sulfonate-Mediated Self-Assembly of Silk Particles from Formic Acid Solutions into Robust Films.

Polymers·2025
Same journal

Linking Local Water Electrostatic Potentials to Measured Hydrogen Evolution Onset in Aqueous Electrolytes.

The journal of physical chemistry letters·2026
Same journal

Microsolvation Redirects Electron-Induced Chemistry in Nucleobases.

The journal of physical chemistry letters·2026
Same journal

Interfacial Microenvironment Effects on the Mechanism of Photocatalytic Methanol Conversion for Hydrogen Evolution.

The journal of physical chemistry letters·2026
Same journal

Noncovalent Interactions in Protein-Ti Binding: Titan Bonds at Work.

The journal of physical chemistry letters·2026
Same journal

Partial Phase Remixing of Segregated Mixed Halide Perovskite Nanocrystals Induced by an Instant Change in an External Electric Field.

The journal of physical chemistry letters·2026
Same journal

Pressure-Driven Dissociation of a Kr Clathrate in the Presence of Colloids.

The journal of physical chemistry letters·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

7.9K

Destabilizing Interactions in Human Telomeric G-Quadruplex Multimers.

Luca Bertini1, Mattia Trapella1, Deniz Mostarac2

  • 1Department of Physics and Geology, University of Perugia, via Alessandro Pascoli, 06123 Perugia, Italy.

The Journal of Physical Chemistry Letters
|May 23, 2025
PubMed
Summary
This summary is machine-generated.

G-Quadruplexes in human telomeres form complex structures. Destabilizing interactions limit stacking, suggesting a helix-coil model for G-Quadruplex arrangements in long DNA sequences.

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

384
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

9.6K

Related Experiment Videos

Last Updated: Jun 12, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

7.9K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

384
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

9.6K

Area of Science:

  • Biophysics
  • Genetics
  • Molecular Biology

Background:

  • G-Quadruplexes are secondary structures in G-rich nucleic acids.
  • Human telomeres feature extended overhangs with G-Quadruplex formation potential.
  • These structures play crucial roles in regulating biological processes.

Purpose of the Study:

  • To investigate the arrangement of G-Quadruplexes in long telomeric sequences.
  • To understand the interactions and conformational preferences of G-Quadruplexes in telomeres.

Main Methods:

  • Utilized small-angle X-ray scattering (SAXS) for experimental guidance.
  • Employed extremely coarse-grained simulations for modeling.

Main Results:

  • Observed significant destabilizing interactions between G-Quadruplexes.
  • Found that stacked conformations are less prevalent than anticipated.
  • Proposed a helix-coil model to describe stacking-unstacking equilibrium.

Conclusions:

  • G-Quadruplex arrangements in long telomeric sequences are influenced by destabilizing interactions.
  • The helix-coil model can predict G-Quadruplex multiplet occurrences in telomeres.