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Hydrolase activity in acute otitis media with effusion.

W F Diven, R H Glew, K L LaMarco

    The Annals of Otology, Rhinology, and Laryngology
    |July 1, 1985
    PubMed
    Summary
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    This study found elevated neuraminidase enzyme levels in middle ear effusions (MEE) from acute otitis media (AOM) patients, particularly when Streptococcus pneumoniae was present. Enzyme activity did not correlate with bacterial infectivity.

    Area of Science:

    • Biochemistry
    • Otolaryngology
    • Microbiology

    Background:

    • Chronic otitis media with effusion (OME) shows elevated hydrolytic and oxidative enzymes in middle ear effusions (MEE).
    • Lysosomal hydrolase activity in MEE from acute OME requires further investigation.

    Purpose of the Study:

    • To quantify lysosomal hydrolase levels in MEE from acute OME patients.
    • To compare enzyme activity between purulent and serous effusions.
    • To investigate the relationship between enzyme activity, effusion type, and bacterial culture results.

    Main Methods:

    • Analysis of MEE samples from acute OME patients.
    • Assay of lysosomal hydrolases: acid phosphatase, alpha-mannosidase, beta-galactosidase, beta-glucuronidase, hexosaminidase, and neuraminidase.

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  • Correlation with bacterial culture status (culture-positive vs. culture-negative) and effusion characteristics (purulent vs. serous).

    • Main Results:

    • No significant differences in mean lysosomal hydrolase levels between purulent and serous acute OME effusions.
    • Mean enzyme activities were higher in culture-positive than culture-negative effusions.
    • Significantly higher neuraminidase activity was found in culture-positive effusions, especially those with Streptococcus pneumoniae (78% detection rate).

    Conclusions:

    • Neuraminidase is a potential biomarker in acute OME, particularly for Streptococcus pneumoniae infections.
    • Enzyme levels in MEE do not directly correlate with bacterial infectivity in vitro.
    • Further research into the role of specific enzymes in OME pathogenesis is warranted.