Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydroxytyrosol Modulates Arachidonic Acid Metabolism and Purine Catabolism in Individuals with Prediabetes: An Untargeted Metabolomics Study in a Randomized Controlled Trial.

Antioxidants (Basel, Switzerland)·2026
Same author

Assessment of European health professionals' educational needs in basic principles of geriatric medicine: a focus group qualitative analysis from the PROGRAMMING COST Action 21122.

European geriatric medicine·2026
Same author

Testing the Island Effect in a Highly Mobile Pollinator: Wing Morphological Divergence in <i>Euglossa mixta</i> from Continental and Insular Panama.

Animals : an open access journal from MDPI·2026
Same author

Scaling the manufacture of adipose tissue-derived mesenchymal stromal cells: integrated bioreactor workflows from inoculation to harvest without the need of seed train.

BMC biotechnology·2026
Same author

Wing Shape Fluctuating Asymmetry in Flies: Insights into Environmental and Public Health Risk.

Animals : an open access journal from MDPI·2025
Same author

Identifying new blood biomarkers of neuroplasticity associated with rehabilitation outcomes after stroke.

Scientific reports·2025

Related Experiment Video

Updated: Sep 20, 2025

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.1K

Low Intensity Pulsed Ultrasounds Modulate Adipose Stem Cells Differentiation.

Tamara Fernández-Marcelo1, Alba Calero1, Beatriz de Lucas2

  • 1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Universidad Complutense de Madrid, Madrid, Spain.

Stem Cell Reviews and Reports
|May 23, 2025
PubMed
Summary

Low-intensity pulsed ultrasound (LIPUS) minimally impacts stem cell proliferation but significantly blocks adipogenesis and reduces inflammation in adipose stem cells (ASCs). This suggests LIPUS may prevent ASCs from contributing to obesity.

Keywords:
AdiponectinAdipose-derived stem cellsInflammationLow-intensity pulsed ultrasoundObesity

More Related Videos

Innovative Adipose Tissue Fractionation for Transforming Fat into Specialized Components
04:36

Innovative Adipose Tissue Fractionation for Transforming Fat into Specialized Components

Published on: July 11, 2025

686
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.4K

Related Experiment Videos

Last Updated: Sep 20, 2025

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.1K
Innovative Adipose Tissue Fractionation for Transforming Fat into Specialized Components
04:36

Innovative Adipose Tissue Fractionation for Transforming Fat into Specialized Components

Published on: July 11, 2025

686
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.4K

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cell Biology

Background:

  • Low-intensity pulsed ultrasound (LIPUS) is a non-invasive therapeutic modality.
  • Adipose stem cells (ASCs) are crucial in adipose tissue biology and disease.
  • Understanding LIPUS effects on ASCs is vital for therapeutic applications.

Purpose of the Study:

  • To investigate the physiological and molecular effects of LIPUS on mouse and human ASCs.
  • To evaluate LIPUS impact on ASC differentiation, migration, and inflammatory profiles.
  • To assess LIPUS effects on ex vivo adipose tissue explants.

Main Methods:

  • Cell culture of mouse and human ASCs.
  • LIPUS stimulation and subsequent proliferation/apoptosis assays.
  • RNA-sequencing (RNA-seq) for gene expression analysis.
  • Migration assays and adipogenic differentiation studies.
  • Ex vivo adipose tissue explant experiments.

Main Results:

  • LIPUS (1-minute stimulation) did not alter ASC proliferation, apoptosis, morphology, or cell growth.
  • RNA-seq revealed differential gene expression in stemness, metabolism, and chemokine signaling pathways.
  • ASC migration was slightly enhanced, while stemness properties were maintained.
  • LIPUS significantly inhibited adipocyte differentiation and reduced inflammatory secretions in ASCs and adipose tissue.
  • Reduced lipid content was observed in LIPUS-treated adipose tissue explants.

Conclusions:

  • LIPUS effectively blocks adipogenesis and reduces the inflammatory profile of ASCs.
  • LIPUS treatment of ASCs may prevent their differentiation into mature adipocytes, mitigating inflammation.
  • These findings suggest LIPUS as a potential therapeutic strategy to limit adipose tissue expansion and associated inflammation in obesity.