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'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
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Bacteriophage-Gated Optical Sensor for Bacteria Detection.

Busra Canan Aslan1,2, Esra Ekiz3, Emine Kubra Tayyarcan3

  • 1Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Ankara 06560, Türkiye.

Analytical Chemistry
|May 23, 2025
PubMed
Summary

A new bacteriophage-gated sensor rapidly detects Escherichia coli (E. coli) with high sensitivity. This innovative tool offers a low-cost, accessible method for bacterial diagnostics, crucial for public health and environmental monitoring.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Microbiology

Background:

  • Antimicrobial resistance necessitates rapid, accessible bacterial diagnostics.
  • Current methods for detecting bacterial infections can be slow and resource-intensive.
  • There is a critical need for point-of-care diagnostic tools for bacterial pathogens.

Purpose of the Study:

  • To develop a novel bacteriophage-gated sensor for the selective detection of Escherichia coli.
  • To create a rapid, sensitive, and user-friendly diagnostic tool for bacterial identification.
  • To assess the sensor's performance in real-world environmental samples.

Main Methods:

  • Utilized bacteriophages as gatekeepers on mesoporous silica particles containing a fluorescent dye (sulforhodamine B).
  • Engineered the sensor to release the dye upon specific interaction with E. coli, generating a fluorescence signal.
  • Integrated the sensor into a Point-of-Care Testing (POCT) device for rapid analysis.

Main Results:

  • Achieved a highly sensitive detection limit of 10^1 CFU/mL for E. coli within 5 minutes.
  • Demonstrated naked-eye fluorescence detection using a simple LED lamp, indicating ease of use.
  • Validated the sensor's accuracy in real water samples, showing consistency with standard culture methods.

Conclusions:

  • The bacteriophage-gated sensor is a low-cost, rapid, and reliable tool for E. coli detection.
  • The sensor meets ASSURED criteria for POCT applications, including affordability and sensitivity.
  • Potential applications include environmental monitoring and clinical diagnostics, addressing public health concerns.