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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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Transcriptomic profiling revealed the regulatory pathways and key genes associated with cold tolerance in two eel
Jing Liu1, Yantao Liu1, Tianwei Liu1
1National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, College of Marine Sciences and Technology, Zhejiang Ocean University, Zhoushan, 316022, China.
Journal of Thermal Biology
|May 23, 2025
Summary
Two eel goby species show different cold tolerance. This study identified key genes and pathways, like PPAR signaling, involved in cold adaptation, revealing molecular mechanisms for survival in low temperatures.
Area of Science:
- * Marine Biology
- * Molecular Biology
- * Evolutionary Biology
Background:
- * Closely related eel goby species exhibit varied tolerance to low temperatures.
- * The underlying molecular mechanisms of this cold tolerance divergence are not well understood.
Purpose of the Study:
- * To identify key molecular pathways and genes associated with cold tolerance in two eel goby species using a comparative transcriptomic approach.
- * To elucidate the genetic basis of interspecific divergence in cold tolerance.
Main Methods:
- * Comparative transcriptomic analysis of two eel goby species (O. lacepedii and O. rebecca) under control (23°C) and cold stress (15°C and 11°C) conditions.
- * Differentially expressed gene analysis using transcriptome profiling and Venn diagrams.
- * Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses.
- * Weighted Gene Co-expression Network Analysis (WGCNA) to identify hub genes.
Main Results:
- * Transcriptome profiling identified differentially expressed genes linked to interspecific cold tolerance divergence.
- * GO and KEGG analyses revealed activation of cellular homeostasis, PPAR signaling pathway, cellular respiration, and oxidative phosphorylation during cold response.
- * WGCNA highlighted PPARGC1A and α-tubulin as potential hub genes contributing to high cold tolerance in O. lacepedii.
Conclusions:
- * The study identifies crucial molecular pathways and genes, including PPARGC1A and α-tubulin, involved in eel goby cold tolerance.
- * Findings provide insights into the molecular mechanisms driving the evolution of cold adaptation in eel gobies.
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