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Updated: May 6, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
Published on: February 6, 2020
Establishment of a novel refinement workflow for oligopeptides containing branched-chain amino acids with
Jinqiu Liu1, Bingxue Jiang1, Yaoguang Chang1
1State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China.
Abstract:
Peptides containing branched-chain amino acids (BCAAs) are recognized for their diverse physiological functions; however, effective methods for their target refinement remain underdeveloped. This study introduced a novel approach utilizing the cavity structure of cyclodextrins, along with specific adsorption (50% ethanol, 50°C) and crystallization conditions (75% ethanol, 4°C), to selectively isolate BCAA-containing oligopeptides from Antarctic krill. Following refinement, the relative BCAA content was elevated to 1.70-fold compared to the original peptide mixture, with the number and abundance of BCAA-containing peptides reaching 95.00% and 94.96%, respectively. Principal component and spectral analysis demonstrated that initial ethanol concentration and cyclodextrin cavity size significantly influenced the peptidome profiles by modulating peptide-cyclodextrin interactions. This study provides a comprehensive exploration of the molecular interactions between peptides and cyclodextrins, highlighting a novel application of cyclodextrins for target peptide isolation and offering deeper insights into the principles governing cyclodextrin inclusion.
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