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Innovating stomach fluke identification: an integrative approach combining Micro-CT imaging and molecular tools.
Ruben Schols1, Arnaud Henrard2, Jonathan Brecko3
1Laboratory of Aquatic Biology, Microbiome EcoEvo unit, KU Leuven Kulak, E. Sabbelaan 53, 8500 Kortrijk, Belgium; Department of Biology, Royal Museum for Central Africa, Leuvensesteenweg 13, 3080 Tervuren, Belgium.
Micro-computed tomography (micro-CT) offers a non-destructive method for identifying amphistome parasites, crucial for biodiversity conservation. This advanced imaging technique aids in parasite taxonomy and reveals new species records from hippos.
Area of Science:
- Parasitology
- Biodiversity studies
- Taxonomy
Background:
- Biodiversity loss necessitates improved species identification, especially for parasites like amphistomes.
- Traditional identification methods (SEM, sectioning) are destructive, labor-intensive, and require specialized expertise.
- Accurate amphistome identification is hindered by undescribed species and scarce reference sequences.
Purpose of the Study:
- To evaluate micro-computed tomography (micro-CT) as a non-destructive alternative for amphistome identification.
- To compare micro-CT with traditional methods (sectioning, SEM) and molecular barcoding.
- To advance integrative taxonomy and address challenges in amphistome species identification.
Main Methods:
- Micro-computed tomography (micro-CT) imaging of three amphistome species from Hippopotamus amphibius.
- Comparison of micro-CT data with Scanning Electron Microscopy (SEM), median sagittal sections, and molecular barcoding.
- High-resolution imaging and designation of lectotypes/paralectotypes for type specimens.
Main Results:
- Micro-CT imaging proved effective for non-destructive amphistome identification.
- The study identified Carmyerius aff. chabaudi as a new species record for the common hippopotamus.
- A taxonomic revision of the genus Carmyerius is suggested, focusing on new diagnostic characters.
- New lectotypes and paralectotypes were designated for Carmyerius cruciformis and Gigantocotyle gigantocotyle.
Conclusions:
- Micro-computed tomography (micro-CT) is a powerful, non-invasive tool for amphistome identification.
- This method facilitates the study of fragile natural history collections and supports integrative taxonomy.
- The findings contribute to better understanding amphistome diversity and conservation efforts.
