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FIBS-enabled Noninvasive Metabolic Profiling

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Published on: February 3, 2014

A biochemical sensor with continuous extended stability in vivo.

Yihang Chen^1,2, Kaiyu X Fu^2,3,4, Renee Cotton⁵

¹Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.

Nature Biomedical Engineering

|May 23, 2025

Summary

Researchers developed a novel synthetic biosensor inspired by intestinal mucosa. This biomimetic sensor offers continuous, real-time in vivo detection of target molecules, overcoming challenges like biofouling.

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Published on: July 16, 2018

Area of Science:

Biomaterials Science
Bioengineering
Analytical Chemistry

Background:

In vivo biosensing is challenging due to biofouling, probe degradation, and signal drift.
Intestinal mucosa provides a model for protecting host cells in a complex biological environment.

Purpose of the Study:

To develop a synthetic biosensor capable of continuous, real-time in vivo analyte detection.
To create a robust biosensor system that overcomes common in vivo limitations.

Main Methods:

Designed a biomimetic multicomponent sensor with a hierarchical nano-bio interface.
Incorporated a three-dimensional bicontinuous nanoporous structure, polymer coating, and aptamer switches.
Tested sensor stability and performance in undiluted serum in vitro and in vivo in free-moving rats.

Main Results:

The sensor demonstrated stability for at least 1 month in serum and 1 week in rat blood vessels.
Maintained over 50% of baseline signal with reproducible calibration curves.
Successfully tracked pharmacokinetics intravenously in real time for 4 days in rats.

Conclusions:

The developed synthetic biosensor provides a generalizable design for continuous in vivo operation.
This biomimetic approach enhances biosensor stability and functionality in complex biological settings.
The system offers a promising foundation for long-term in vivo monitoring applications.