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piR-RCC Suppresses Renal Cell Carcinoma Progression by Facilitating YBX-1 Cytoplasm Localization

Ruyue Wang1, Fan Li1, Yudong Lin1

  • 1Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 24, 2025
PubMed

Insights

A novel PIWI-interacting RNA (piRNA), piR-RCC, inhibits renal cell carcinoma (RCC) progression by targeting the YBX-1/EHF pathway. Biomimetic nanoparticles deliver piR-RCC, offering a potential new therapy for RCC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biotechnology

Background:

  • PIWI-interacting RNAs (piRNAs) are small non-coding RNAs implicated in various pathologies, including cancer.
  • The specific role of piRNAs in renal cell carcinoma (RCC) progression remains largely uncharacterized.

Purpose of the Study:

  • To investigate the function and mechanism of piRNAs in RCC progression.
  • To identify a novel piRNA that can impede RCC development.
  • To develop a targeted delivery system for potential therapeutic applications.

Main Methods:

  • Identification and characterization of a down-regulated piRNA (piR-RCC) in RCC.
  • In vitro and in vivo studies to assess piR-RCC's effect on RCC progression.
  • Investigation of the molecular mechanism involving Y-box binding protein 1 (YBX-1) and ETS homologous factor (EHF).
  • Construction and evaluation of a biomimetic nanoparticle platform for targeted piR-RCC delivery.

Main Results:

  • piR-RCC was found to be down-regulated in RCC and inhibits RCC progression.
  • piR-RCC directly interacts with YBX-1, preventing its phosphorylation and nuclear translocation.
  • This interaction leads to enhanced EHF expression, suppressing RCC proliferation and metastasis.
  • Biomimetic nanoparticles successfully delivered piR-RCC to renal tumors in mice, inhibiting RCC progression.

Conclusions:

  • The piR-RCC/YBX-1/EHF signaling axis plays a critical role in inhibiting RCC progression.
  • piR-RCC represents a potential therapeutic target for renal cell carcinoma.
  • Biomimetic nanoparticle delivery of piR-RCC offers a promising strategy for RCC treatment.
Keywords:
PIWI‐interacting RNAsSTAT3YBX‐1nanoparticlerenal cell carcinoma

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