Entomopathogenic nematodes: Survival, virulence and immunity

As entomopathogenic nematodes (EPNs) are used as biological control agents, their survival and persistence are crucial to ensure success in application against insect pests. The survival of Heterorhabditis and Steinernema species is dependent on abiotic and biotic factors in the environment. Abiotic stress environments such as desiccation, temperature, and ultraviolet radiation (UV) severely impact their performance on field. EPNs produce and secrete effector molecules during the early stages of infection to interfere with the molecular mechanisms that control the insect innate immune function. Also, EPN effectors facilitate the subsequent release and spread of their symbiotic bacteria within the host. Hence, a comprehensive understanding of the underlying survival and virulence mechanisms enabling protection against environmental conditions and insect host immune responses is imperative to realistically enhance their performance on field. Thus, identifying key players regulating EPN survival, virulence and immunity could invariably contribute towards developing more robust, reliable solutions and application strategies including genetic tools and formulation technologies.