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Updated: Sep 20, 2025
Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
Published on: May 30, 2015
Accurate detection of pathologic α-synuclein in CSF, skin, olfactory mucosa, and urine with a uniform seeding
Remarh Bsoul1, Oskar H McWilliam2, Gunhild Waldemar2,3
1Danish Reference Center for Prion Diseases, Department of Pathology, Copenhagen University Hospital, Copenhagen, Denmark.
Abstract:
Currently, early diagnosis of dementia with Lewy bodies (DLB) is based on clinical criteria, which is challenging due to overlapping symptoms with other neurodegenerative diseases. Seeding amplification assays, detecting minute amounts of disease causing α-synuclein (αSynD), are emerging as a promising diagnostic tool for α-synucleinopathies including DLB and Parkinson's disease. This study aimed to test whether the same seeding amplification assay established for αSynD detection in cerebrospinal fluid (CSF) could be applied to other biospecimens, including skin, olfactory mucosa, saliva, and urine, obtained from the same patients. A total of 31 patients with probable DLB and 53 healthy controls were recruited. When evaluating the assays' applicability to different biospecimens, only those collected from participants with a positive CSF αSynD result were considered. Seeding amplification assay results were evaluated based on the αSynD amplification rate over 48 h and the value of the area under the curve. The sensitivity and specificity were 94% and 98% for skin, 47% and 100% for olfactory mucosa, and 22% and 100% for urine, respectively for the CSF positive DLB and healthy controls. αSynD was undetectable in saliva. Cohen's Kappa analysis (κ) showed almost perfect agreement between CSF and skin assays (κ = 0.86) but slight to no agreement for CSF versus olfactory mucosa (κ = 0.12) and urine (κ = 0.094). In summary, the seeding amplification assay established for αSynD detection in CSF demonstrated comparable diagnostic performance in minimally invasive skin biopsies. Olfactory mucosa, saliva, and urine sample preparation pose technical challenges resulting in the established assays' low diagnostic accuracy, for now, limiting their use in diagnostics. Nevertheless, the proof-of-concept for αSynD detection in urine expands the potential for non-invasive diagnostics of α-synucleinopathies in the future.
