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Updated: Jul 13, 2026
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
Published on: April 14, 2016
Protocol for assessing the structural architecture, integrity, and cellular composition of murine bone ex vivo
Jonathan W Lewis1, Kathryn Frost1, Georgiana Neag1
1Department of Inflammation and Ageing, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Birmingham, UK.
None:
Understanding changes in murine bone is essential to comprehend bone homeostasis and the role of therapeutics in bone diseases. Here, we present a protocol to assess the structural architecture, integrity, and cellular composition of murine bone ex vivo. We describe steps for imaging and analyzing bone by micro-computed tomography (CT) and functionally testing bone strength through three-point bending. We then detail procedures for histology and analysis of calcein labeling. For complete details on the use and execution of this protocol, please refer to Lewis et al.1.
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