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Differential systemic antibody responses to Mycoplasma synoviae MSPA variants after vaccination and/ or field
Oluwadamilola S Omotainse1, Amir H Noormohammadi1, Nathan Jeffery1
1Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Werribee, VIC, Australia.
Abstract:
Mycoplasma synoviae has a major immunodominant surface protein VlhA (variable lipoprotein and haemagglutinin) which undergoes post-translational cleavage to form two separate proteins, MSPA and MSPB. Both these proteins are targets of the host immune response undergo high frequency antigenic variation and are thought to contribute to the pathogenesis of M. synoviae infection. In order to assess variation of MSPA in response to vaccination and/ or field challenge host systemic humoral responses after inoculation with M. synoviae strain 86079/7NS (7NS), vaccine strain MS-H and/or field strain 94011/v-18d (v-18d) were evaluated against eight variable regions of MSPA derived from M. synoviae type strain WVU-1853. At 21 days post-infection, chickens inoculated with 7NS had significantly higher antibody levels against MSPA B3 and B4 compared to those inoculated with the vaccine strain MS-H. Also, at 21 days post-challenge, chickens which had been inoculated with the MS-H vaccine strain prior to challenge had significantly lower antibody responses against MSPA C2 compared to those which had not been. This suggests that variants of MSPA are differentially expressed after vaccination and/ or challenge and antibodies against these variants may be strain dependent. The results of this study have implications for the differentiation of serological responses after M. synoviae vaccination and field challenge.
Insights
Antibodies against Mycoplasma synoviae surface protein variants are strain-dependent. This finding impacts differentiating serological responses after vaccination and field challenges in chickens.
Area of Science:
- Veterinary immunology
- Microbiology
Background:
- Mycoplasma synoviae infection poses a significant threat to poultry health.
- The VlhA protein, comprising MSPA and MSPB, is a key target of the host immune response and undergoes antigenic variation, contributing to M. synoviae pathogenesis.
Purpose of the Study:
- To evaluate host systemic humoral responses against variable regions of MSPA following vaccination and/or field challenge.
- To assess the strain-specific nature of antibody responses to MSPA variants.
Main Methods:
- Chickens were inoculated with M. synoviae strains (7NS, MS-H, v-18d).
- Systemic humoral responses were measured against eight variable regions of MSPA.
- Antibody levels were assessed at 21 days post-infection and post-challenge.
Main Results:
- Chickens inoculated with strain 7NS showed higher antibody levels against MSPA B3 and B4 compared to those inoculated with vaccine strain MS-H.
- Chickens vaccinated with MS-H prior to challenge exhibited lower antibody responses against MSPA C2 compared to unvaccinated controls.
- Antibody responses against MSPA variants were found to be strain-dependent.
Conclusions:
- Variants of MSPA are differentially expressed following vaccination and/or field challenge.
- Antibodies generated against MSPA variants may be strain-specific, impacting serological differentiation.
- These findings have implications for distinguishing vaccination-induced serological responses from field challenge responses in M. synoviae infections.
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