Distribution and chromatographic characterization of neuromedin B-like immunoreactivity in the human spinal cord

The quantitative regional distribution of neuromedin B-like immunoreactivity in normal postmortem human spinal cord was studied by a specific radioimmunoassay. Neuromedin B-like immunoreactivity was found in highest concentration in the dorsal part of the sacral cord. Chromatographic analyses by gel permeation and reverse-phase high-pressure liquid chromatography revealed two major peaks of neuromedin B-like immunoreactivity and the prevalent molecular form, approx. 90% of the total immunoreactivity, was chromatographically identical to synthetic porcine neuromedin B.