Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrogen ion buffering during complete brain ischemia.

R P Kraig, W A Pulsinelli, F Plum

    Brain Research
    |September 9, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Behavioural improvements with thalamic stimulation after severe traumatic brain injury.

    Nature·2007
    Same author

    fMRI reveals large-scale network activation in minimally conscious patients.

    Neurology·2005
    Same author

    Optimization of multiplexed bead-based cytokine immunoassays for rat serum and brain tissue.

    Journal of neuroscience methods·2004
    Same author

    Neuroprotection in acute ischemic stroke.

    JAMA·2001
    Same author

    Astrocytic clasmatodendrosis in hippocampal organ culture.

    Glia·2001
    Same author

    Transient global ischemia in rats yields striatal projection neuron and interneuron loss resembling that in Huntington's disease.

    Experimental neurology·2000
    Same journal

    Targeting neurodevelopmental miR132-3p promotes neuroprotection and axon regeneration after optic nerve injury in mice.

    Brain research·2026
    Same journal

    Variability in acoustic startle response and prepulse inhibition across adulthood in Fragile X messenger ribonucleoprotein 1 knockout mice.

    Brain research·2026
    Same journal

    Transcriptome-guided modeling reveals insulin-related metabolic dysfunction in SCA3 mouse cerebellum.

    Brain research·2026
    Same journal

    Intranasal stromal cell-derived factor-1α mitigates parkinsonian deficits via dual modulation of neuroinflammation and gut microbiota in MPTP-induced models.

    Brain research·2026
    Same journal

    Emotions, the amygdala, and the right hemisphere.

    Brain research·2026
    Same journal

    Electroacupuncture treatment enhances hippocampal growth hormone level and restores mitochondrial function in vascular dementia rats.

    Brain research·2026
    See all related articles

    Brain interstitial [H+] changes during ischemia show a step-function relationship with tissue lactate, not a linear one. Astroglial ion transport likely explains this, preventing normal ion regulation and leading to brain infarction.

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Physiology

    Background:

    • Ischemia leads to changes in brain interstitial [H+] (intracellular and extracellular hydrogen ion concentration).
    • Understanding the relationship between [H+]o and tissue lactate is crucial for quantifying these changes during ischemia.

    Purpose of the Study:

    • To quantify [H+] changes in the brain during ischemia.
    • To describe the relationship between interstitial [H+] ([H+]o) and peak tissue lactate after cardiac arrest.

    Main Methods:

    • Utilized H+-selective microelectrodes.
    • Employed enzyme fluorometric techniques.

    Main Results:

    • Observed a step-function relationship between [H+]o and tissue lactate, deviating from expected linear titration.

    Related Experiment Videos

  • Found that at higher blood glucose levels, brain lactate accumulated significantly, accompanied by a substantial rise in [H+]o.
  • Noted that [H+]o reached a steady-state, not equilibrium, with other brain compartments despite lactate accumulation.

    • Conclusions:

    • Astroglial ion-transport characteristics likely explain the observed [H+]o to tissue lactate relationship during ischemia.
    • Brain infarction may develop when brain cell plasma membranes lose their ion-transport regulatory capacity.