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Updated: Aug 5, 2026

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
Published on: June 23, 2012
Protocol to perform multiplexed assays of variant effect using curated loci prime editing
Carina G Biar1, Nicholas Bodkin2, Gemma L Carvill2
1Ken and Ruth Davee Department of Neurology, Northwestern Feinberg School of Medicine, Chicago, IL 60611, USA; Genome Sciences, University of Washington, Seattle, WA 98195, USA.
None:
Multiplexed assays of variant effect (MAVEs) perform simultaneous characterization of many variants. Here, we present a protocol to perform MAVEs using curated loci prime editing (cliPE), an accessible experimental pipeline that enables prime editing of a target gene. We describe steps for designing prime editing reagents, screening for genome editing efficiency, selecting a pool of cells edited to harbor different genetic variants, and sequencing. Lastly, we detail procedures for performing enrichment analysis to identify variants with normal or aberrant activity.
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