Related Experiment Video
Updated: May 10, 2026
06:52
Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin
Published on: April 30, 2019
18.4K
Role of Slc7a11 in Bleomycin-Induced Lung Injury
Jeffrey D Ritzenthaler1,2, Edilson Torres-Gonzalez1,2, Yvonne Janssen-Heininger3
1Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Thomas Jefferson University, Philadelphia, PA, USA.
Lung
|May 26, 2025
Summary
Altering the expression of Slc7a11, a regulator of plasma redox potential (Eh Cys/CySS), did not impact lung injury in mice. The role of Slc7a11 in lung injury and repair remains uncertain.
Area of Science:
- Biochemistry
- Cell Biology
- Pulmonary Medicine
Background:
- Oxidative stress is crucial in lung injury and repair processes.
- Plasma redox potential (Eh) of the cysteine/cystine (Cys/CySS) couple influences lung fibroblast behavior.
- Solute carrier family 7, member 11 (Slc7a11) regulates Eh Cys/CySS and fibroblast phenotype in vitro.
Purpose of the Study:
- To investigate the in vivo role of Slc7a11 in lung injury.
- To determine if modulating Slc7a11 expression affects lung injury outcomes in a mouse model.
Main Methods:
- Utilized C57Bl6 mice exposed to bleomycin to induce lung injury.
- Administered pharmacological agents to alter Slc7a11 expression.
- Tested Slc7a11 knockout mice in the bleomycin-induced lung injury model.
Main Results:
- Pharmacological alterations in Slc7a11 expression did not alter bleomycin-induced lung injury.
- Slc7a11 knockout mice showed no significant changes in lung injury compared to controls.
- In vivo findings contrast with previously observed in vitro effects on lung fibroblasts.
Conclusions:
- The study did not find evidence that Slc7a11 modulates lung injury in vivo.
- The role of Slc7a11 in the context of lung injury and repair requires further investigation.
- Current findings suggest Slc7a11's role in lung injury is uncertain despite its in vitro effects.
Related Concept Videos
01:23Cystic Fibrosis: Pathogenesis
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation, but...
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation, but...
01:00Cellular Injury I: Introduction
Cellular injury occurs when a cell cannot maintain homeostasis or adapt to stressors such as hypoxia, toxins, or trauma. Depending on severity and duration, injury may be reversible, allowing recovery, or irreversible, leading to cell death.General Mechanisms of Cell InjuryAlthough causes vary, most cellular injuries arise from a few key mechanisms that disrupt essential functions and often amplify one another. Cell survival depends on the extent and balance of these disturbances.ATP depletion...