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Updated: Aug 6, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
Published on: September 16, 2025
Nondestructive Metabolic Monitoring of Living Organisms by Water-Droplet Extraction and Contact-Free Electrospray
Yuanyi He1,2,3,4, Xiaojie Chen1,2,3, Lecheng Lyu4
1Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, China.
Abstract:
We present a novel tool for nondestructive in vivo metabolite analysis, which we call MetaTube. This method utilizes a high voltage to facilitate efficient ionization while maintaining electrical isolation from living tissues that are sampled. MetaTube achieves a 2-3 order of magnitude improvement in metabolite signal intensity, giving MetaTube the capacity to identify 10-fold more metabolites. These capabilities allow us to profile metabolites of human skin surfaces and monitor metabolite fluctuations of growing bacterial colonies. MetaTube offers a promising strategy to understand in vivo metabolic dynamics and evaluate the metabolic state of living tissues in real time.
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