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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
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Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
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In Vivo Ribosome-Amplified MetaBOlism, RAMBO, Effect Observed by Real Time Pulse Chase, RTPC, NMR Spectroscopy.

Jianchao Yu1, Nicholas Sciolino1, Leonard Breindel1

  • 1Department of Chemistry, University at Albany, State University of New York, Albany, New York 12222, United States.

Biochemistry
|May 27, 2025
PubMed
Summary
This summary is machine-generated.

The Ribosome-Amplified MetaBOlism (RAMBO) effect, previously only seen in vitro, has now been validated in vivo in E. coli. This study confirms ribosomes

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Systems Biology

Background:

  • Quinary interactions involving proteins and ribosomes influence biological activity via the Ribosome-Amplified MetaBOlism (RAMBO) effect.
  • The RAMBO effect has been observed in vitro, but its in vivo validation remained elusive.

Purpose of the Study:

  • To validate the RAMBO effect in vivo using Escherichia coli.
  • To investigate the role of ribosomes in regulating glycolysis through the RAMBO effect.

Main Methods:

  • Real-time pulse chase (RTPC) NMR spectroscopy combined with isotopic flux analysis in E. coli.
  • Utilized the antibiotic chloramphenicol to disrupt pyruvate kinase's quinary structure and the RAMBO effect.

Main Results:

  • The in vivo deactivation of the RAMBO effect by chloramphenicol mirrored in vitro observations.
  • Confirmed the potential role of ribosomes in regulating glycolysis in living cells.

Conclusions:

  • The study successfully validated the RAMBO effect in vivo, demonstrating ribosomes' regulatory role in glycolysis.
  • The developed RTPC-NMR platform offers a noninvasive method for high-resolution metabolic monitoring in various cell types.