Optimizing Whole-Cell Biosensors for the Early Detection of Crop Infections: A Proof-of-Concept Study

Area of Science:

• Agricultural Science
• Microbiology
• Biotechnology

Background:

• Crop spoilage leads to significant postharvest losses.
• Early detection of infections is crucial for maintaining crop quality.
• Current detection methods can be slow or require specialized equipment.

Purpose of the Study:

• To evaluate optimized whole-cell biosensors for real-time detection of crop infections.
• To assess the impact of environmental factors on biosensor performance.
• To demonstrate the feasibility of biosensor application in postharvest monitoring.

Main Methods:

• Genetically engineered luminescent bacterial strains were developed.
• Detection of volatile organic compounds (VOCs) emitted by infected crops.
• Investigation of bacterial uniformity, nutrient supply, and temperature effects.
• Proof-of-concept evaluation under storage-like conditions.

Main Results:

• Lower temperatures (+4 °C) significantly increased sensor sensitivity.
• Optimal conditions prolonged bacterial viability for sustained detection.
• Effective detection of potato infections was demonstrated.
• The biosensor system showed high sensitivity to spoilage-related VOCs.

Conclusions:

• Optimized whole-cell biosensors show promise for real-time infection detection.
• Cold storage conditions are suitable for biosensor operation.
• This technology can enhance postharvest management and reduce food waste.