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The quantification of human IgG subclasses in reference preparations.

F Klein, F Skvaril, R Vermeeren

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |August 15, 1985
    PubMed
    Summary
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    Quantitative determination of immunoglobulin G (IgG) subclasses showed variable precision across different subclasses. Mass units and WHO reference preparation 67/97 are recommended for accurate IgG subclass quantification.

    Area of Science:

    • Immunology
    • Clinical Chemistry
    • Laboratory Medicine

    Background:

    • Accurate quantification of immunoglobulin G (IgG) subclasses is crucial for diagnosing and monitoring various immune-related conditions.
    • Standardization of IgG subclass measurement methods is needed to ensure reliable and comparable results across different laboratories.
    • Previous studies highlighted variability in IgG subclass determination, necessitating further investigation into optimal quantification strategies.

    Purpose of the Study:

    • To evaluate the feasibility of quantitative determination of IgG subclasses using polyclonal monospecific antisera and various calibrators.
    • To assess the precision and reliability of IgG subclass quantification across three participating laboratories.
    • To propose a standardized reference preparation and target values for mass unit determination of IgG subclasses.

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    Main Methods:

    • Three laboratories participated in the study, employing polyclonal monospecific antisera for IgG subclass analysis.
    • Different types of calibrators were utilized to assess IgG subclass levels in three World Health Organization (WHO) reference preparations.
    • Quantitative determination of IgG subclasses (IgG 1, IgG 2, IgG 3, and IgG 4) was performed.

    Main Results:

    • Satisfactory precision was achieved for the quantification of IgG 1 subclass.
    • Less precise quantification was observed for IgG 2, IgG 3, and IgG 4 subclasses.
    • Precision was influenced by antisera for IgG 1, IgG 2, and IgG 4, while calibrators primarily affected IgG 3 precision.
    • Mass units were found to be preferable over relative units for IgG subclass determination.

    Conclusions:

    • WHO reference preparation 67/97 is proposed as a suitable reference for determining IgG subclasses in mass units.
    • Recommended target values for WHO 67/97 are 5.0 g/l for IgG 1, 2.6 g/l for IgG 2, 0.4 g/l for IgG 3, and 0.5 g/l for IgG 4.
    • These target values align with previous WHO estimates for total IgG levels, supporting their validity.